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S1-Tag Antibody, AbBy Fluor™ 488 Conjugated

Product Specifications

Background

Epitope tagging is a recombinant DNA method by which a protein encoded by a cloned gene is made immunoreactive to a known antibody. Major areas ofapplication include monitoring protein expression, localizing proteins at thecellular and subcellular levels, and protein purification, as well as the analysis of protein topology, dynamics and interactions. Due to their small size, theyare unlikely to affect the tagged protein's biochemical properties.S1 tag is an epitope tag composed of a nine-residue peptide, NANNPDWDF, derived from the hepatitis B virus preS1 region. Epitope tags consisting ofshort sequences recognized by well-characterizated antibodies have beenwidely used in the study of protein expression in various systems.

Synonyms

S1 Tag; S1Tag; NANNPDWDF

Host

Rabbit

Cross Reactivity

Species independent

Target

S1-Tag

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 488

Source

KLH conjugated synthetic peptide S1-Tag

Applications

WB

Purification

Purified by Protein A.

Excitation Emission

499nm/519nm

Concentration

1µg/µl

Dilution

WB (1:300-5000)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Cross Reactivity Details

S1-Tag

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