Phospho-MYPT1 (Thr853) Polyclonal Antibody

Product Specifications

Background

Key regulator of protein phosphatase 1C (PPP1C) . Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

Synonyms

MBS; M130; MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit; PPP1R12A

Gene ID

4659

Swiss Prot

O14974

Modification Site

Thr853

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

Phospho-MYPT1 (Thr853)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

KLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of Thr853

Applications

ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Specificity

This phosphorylation site is homologous to that of Thr852 in Mouse and Thr855 in Rat.

Gene ID URL

4659

Predicted Cross Reactivity

Rat, Dog, Cow, Horse, Chicken, Rabbit

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