Phospho-c-Jun (Ser63) Polyclonal Antibody, AbBy Fluor™ 750 Conjugated

Product Specifications

Background

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.

Synonyms

AP1; AP-1; c-Jun; Transcription factor AP-1; Activator protein 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUN

Gene ID

3725

Swiss Prot

P05412

Modification Site

Ser63

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-c-Jun (Ser63)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 750

Source

KLH conjugated synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Ser63

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

749nm/775nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

3725

Predicted Cross Reactivity

Dog, Cow, Sheep, Pig, Rabbit

Available Sizes

Curated Selection

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