Phospho-IRS1 (Ser636 + Ser639) Polyclonal Antibody

Product Specifications

Background

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity) .

Synonyms

HIRS-1; Insulin receptor substrate 1; IRS-1; IRS1

Gene ID

3667

Swiss Prot

P35568

Modification Site

Ser636 + Ser639

Cellular Locus

Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-IRS1 (Ser636 + Ser639)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Ser636/639 [PM (p-S) PK (p-S) VS]

Applications

IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

IHC-P (1:200-400), IHC-F (1:100-500), IF (), Flow-Cyt (1ug/Test), ICC/IF (1:50-100)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Specificity

This phosphorylation site is homologous to Ser632 + ser635 in Mouse and Rat IRS1. This protein is known to be highly conserved in this sequences region, thus , this antibody may react with IRS2 when phosphorylated at Ser679 + Ser682 in Human , Ser675 + Ser678 in Mouse, and Ser676 +Ser679 in Rat based on homology.