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Phospho-Histone H3 (Ser10) Polyclonal Antibody, PerCP-Cy5.5 Conjugated

Product Specifications

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Synonyms

H3t; H3.4; H3/g; H3FT; Histone H3.1t; H3/t; HIST3H3

Gene ID

8290

Swiss Prot

Q16695

Modification Site

Ser11

Host

Rabbit

Cross Reactivity

Human, Rat

Target

Phospho-Histone H3 (Ser10)

Clonality

Polyclonal

Isotype

IgG

Conjugation

PerCP-Cy5.5

Source

KLH conjugated synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser11

Applications

FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

482nm/694nm

Concentration

1µg/µl

Dilution

FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

8290

Predicted Cross Reactivity

Mouse, Cow, Pig, Rabbit, Fruit Fly

Curated Selection

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