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Phospho-Histone H3 (Ser10) Polyclonal Antibody, Cy5.5 Conjugated

Product Specifications

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Synonyms

H3t; H3.4; H3/g; H3FT; Histone H3.1t; H3/t; HIST3H3

Gene ID

8290

Swiss Prot

Q16695

Modification Site

Ser11

Host

Rabbit

Cross Reactivity

Human, Rat

Target

Phospho-Histone H3 (Ser10)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy5.5

Source

KLH conjugated synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser11

Applications

FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

675nm/694nm

Concentration

1µg/µl

Dilution

FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

8290

Predicted Cross Reactivity

Mouse, Cow, Pig, Rabbit, Fruit Fly

Curated Selection

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