Phospho-Histone H3 (Ser10) Polyclonal Antibody, Cy3 Conjugated
Product Specifications
Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms
H3t; H3.4; H3/g; H3FT; Histone H3.1t; H3/t; HIST3H3
Gene ID
8290
Swiss Prot
Q16695
Modification Site
Ser11
Host
Rabbit
Cross Reactivity
Human, Rat
Target
Phospho-Histone H3 (Ser10)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy3
Source
KLH conjugated synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser11
Applications
FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
512,550nm/570,615nm
Concentration
1µg/µl
Dilution
FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
8290
Predicted Cross Reactivity
Mouse, Cow, Pig, Rabbit, Fruit Fly
Curated Selection
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