Phospho-Histone H3 (Ser10) Polyclonal Antibody, AbBy Fluor™ 488 Conjugated
Product Specifications
Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms
H3t; H3.4; H3/g; H3FT; Histone H3.1t; H3/t; HIST3H3
Gene ID
8290
Swiss Prot
Q16695
Modification Site
Ser11
Host
Rabbit
Cross Reactivity
Human, Rat
Target
Phospho-Histone H3 (Ser10)
Clonality
Polyclonal
Isotype
IgG
Conjugation
AbBy Fluor™ 488
Source
KLH conjugated synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser11
Applications
FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
499nm/519nm
Concentration
1µg/µl
Dilution
FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
8290
Predicted Cross Reactivity
Mouse, Cow, Pig, Rabbit, Fruit Fly
Curated Selection
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