Phospho-H2AX (Ser139) Polyclonal Antibody, PE-Cy5.5 Conjugated
Product Specifications
Background
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Synonyms
H2AX; H2A.X; H2A/X; Histone H2AX; Histone H2A.X; H2AFX
Gene ID
3014
Swiss Prot
P16104
Modification Site
Ser140
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-H2AX (Ser139)
Clonality
Polyclonal
Isotype
IgG
Conjugation
PE-Cy5.5
Source
KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140
Applications
WB, FCM
Purification
Purified by Protein A.
Excitation Emission
488nm/694nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Specificity
This phosphorylation site is homologous to that of Ser140 in Mouse and Rat.
Gene ID URL
3014
Predicted Cross Reactivity
Dog, Cow, Pig, Horse, Rabbit
Curated Selection
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