Phospho-Histone H1.4 (Thr18) Polyclonal Antibody, APC-Cy5.5 Conjugated
Product Specifications
Background
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) .
Synonyms
H1E; H1.4; H1F4; H1s-4; dJ221C16.5; Histone H1.4; Histone H1b; Histone H1s-4; HIST1H1E
Gene ID
3008
Swiss Prot
P10412
Modification Site
Thr18
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-Histone H1.4 (Thr18)
Clonality
Polyclonal
Isotype
IgG
Conjugation
APC-Cy5.5
Source
KLH conjugated synthetic phosphopeptide derived from human Histone H1.4 around the phosphorylation site of Thr18
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
650nm/694nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Specificity
This phosphorylation site is found in Rat H1C as well, but not Human or Mouse H1C. This phosphorylation site is homologous to Thr18 in Rat H1C/H1D and Mouse H1D. Due to the highly conserved nature of these histones, this antibody may react with these members when phosphorylated at this site.
Gene ID URL
3008
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