Phospho-c-Fos (Thr325) Polyclonal Antibody, RBITC Conjugated

Product Specifications

Background

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

Synonyms

P55; AP-1; C-FOS; Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7; FOS; G0S7

Gene ID

2353

Swiss Prot

P01100

Modification Site

Thr325

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-c-Fos (Thr325)

Clonality

Polyclonal

Isotype

IgG

Conjugation

RBITC

Source

KLH conjugated synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Thr325

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

570nm/595nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

This phosphorylation site is homologous to Thr325 in Mouse and Rat.

Gene ID URL

2353

Predicted Cross Reactivity

Dog, Sheep, Pig, Chicken

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