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Phospho-Cyclin E1 (Thr77) Polyclonal Antibody, Cy5.5 Conjugated

Product Specifications

Background

Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Synonyms

CCNE 1; CCNE; CCNE1; Cyclin E1 antibody Cyclin Es; Cyclin Et; CyclinE; G1/S specic cyclin E; CCNE1_HUMAN.

Gene ID

898

Modification Site

Thr77

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Human

Target

Phospho-Cyclin E1 (Thr77)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy5.5

Source

KLH conjugated synthetic phosphopeptide derived from human Cyclin E around the phosphorylation site of Thr77

Applications

WB, FCM, IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

675nm/694nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.biossantibodies.com/datasheets/bs-3125R-Cy5.5

Gene ID URL

898

Predicted Cross Reactivity

Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit

Curated Selection

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