Phospho-Cyclin B1 (Ser133) Polyclonal Antibody, Cy5 Conjugated
Product Specifications
Background
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].
Synonyms
Cyclin B1 phospho S133; Cyclin B1 Ser133; p-Cyclin B1 S133; p-Cyclin B1 Ser133; p-Cyclin B1 phospho S133; CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specic cyclin B1; G2/mitotic-specic cyclin-B1.
Gene ID
891
Modification Site
Ser133
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human
Target
Phospho-Cyclin B1 (Ser133)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy5
Source
KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser133
Applications
WB, FCM
Purification
Purified by Protein A.
Excitation Emission
625,650nm/670nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
891
Predicted Cross Reactivity
Mouse, Rat, Pig
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