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Phospho-Cyclin B1 (Ser133) Polyclonal Antibody, APC Conjugated

Product Specifications

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].

Synonyms

Cyclin B1 phospho S133; Cyclin B1 Ser133; p-Cyclin B1 S133; p-Cyclin B1 Ser133; p-Cyclin B1 phospho S133; CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specic cyclin B1; G2/mitotic-specic cyclin-B1.

Gene ID

891

Modification Site

Ser133

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human

Target

Phospho-Cyclin B1 (Ser133)

Clonality

Polyclonal

Isotype

IgG

Conjugation

APC

Source

KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser133

Applications

WB, FCM

Purification

Purified by Protein A.

Excitation Emission

650nm/660nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

891

Predicted Cross Reactivity

Mouse, Rat, Pig

Curated Selection

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