Phospho-Cyclin B1 (Ser147) Polyclonal Antibody, RBITC Conjugated
Product Specifications
Background
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].
Synonyms
Cyclin B1 phospho S147; Cyclin B1 Ser147; p-Cyclin B1 S147; p-Cyclin B1 Ser147; p-Cyclin B1 phospho S147; CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specic cyclin B1; G2/mitotic-specic cyclin-B1.
Gene ID
891
Modification Site
Ser147
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human
Target
Phospho-Cyclin B1 (Ser147)
Clonality
Polyclonal
Isotype
IgG
Conjugation
RBITC
Source
KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser147
Applications
WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
570nm/595nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
891
Predicted Cross Reactivity
Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
