Phospho-Cyclin B1 (Ser147) Polyclonal Antibody, Cy5 Conjugated
Product Specifications
Background
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].
Synonyms
Cyclin B1 phospho S147; Cyclin B1 Ser147; p-Cyclin B1 S147; p-Cyclin B1 Ser147; p-Cyclin B1 phospho S147; CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specic cyclin B1; G2/mitotic-specic cyclin-B1.
Gene ID
891
Modification Site
Ser147
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human
Target
Phospho-Cyclin B1 (Ser147)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy5
Source
KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser147
Applications
WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
625,650nm/670nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
891
Predicted Cross Reactivity
Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig
Curated Selection
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