Phospho-Cyclin B1 (Ser147) Polyclonal Antibody, AbBy Fluor™ 680 Conjugated

Product Specifications

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].

Synonyms

Cyclin B1 phospho S147; Cyclin B1 Ser147; p-Cyclin B1 S147; p-Cyclin B1 Ser147; p-Cyclin B1 phospho S147; CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specic cyclin B1; G2/mitotic-specic cyclin-B1.

Gene ID

891

Modification Site

Ser147

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human

Target

Phospho-Cyclin B1 (Ser147)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 680

Source

KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser147

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

679nm/702nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

891

Predicted Cross Reactivity

Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig

Curated Selection

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