Phospho-CRK (Tyr221) Polyclonal Antibody, AbBy Fluor™ 647 Conjugated

Product Specifications

Background

The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.

Synonyms

Adapter molecule crk; Proto-oncogene c-Crk; p38; CRK

Gene ID

1398

Modification Site

Tyr221

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Others

Target

Phospho-CRK (Tyr221)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 647

Source

KLH conjugated synthetic phosphopeptide derived from human Crk p38 around the phosphorylation site of Tyr221 [GP (p-Y) AQ]

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/665nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

1398

Predicted Cross Reactivity

Human, Mouse, Rat, Dog, Horse, Chicken, Rabbit

Curated Selection

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