Phospho-EGFR (Tyr998) Polyclonal Antibody
Product Specifications
Background
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
Synonyms
Epidermal growth factor receptor; p170EGFR; ERBB1; HER1; Avian erythroblastic leukemia viral verbb oncogene homolog; EGF R; EGFR; erbb 1; Erbb; Erbb1; mENA; Oncogene ERBB; Receptor tyrosine protein kinase ErbB1; Urogastrone; wa2; Wa5
Gene ID
1956
Modification Site
Tyr998
Cellular Locus
Cytoplasm, Nucleus, Secreted, Cell membrane
Host
Rabbit
Cross Reactivity
Human
Target
Phospho-EGFR (Tyr998)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic phosphopeptide derived from human EGFR around the phosphorylation site of Tyr998
Applications
WB, ELISA, FCM
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), FCM (1:20-100)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
1956
Predicted Cross Reactivity
Mouse, Rat, Dog, Cow, Pig
Curated Selection
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