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Phospho-DOK2 (Tyr351) Polyclonal Antibody, RBITC Conjugated

Product Specifications

Background

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.

Synonyms

DokR; Frip; Docking protein 2; Dok-related protein; Dok-R; Downstream of tyrosine kinase 2; IL-four receptor-interacting protein; p56 (dok-2) ; Dok2

Gene ID

13449

Swiss Prot

O70469

Modification Site

Tyr351

Cellular Locus

Cytoplasm

Host

Rabbit

Target

Phospho-DOK2 (Tyr351)

Clonality

Polyclonal

Isotype

IgG

Conjugation

RBITC

Source

KLH conjugated synthetic phosphopeptide derived from mouse p56Dok2 around the phosphorylation site of (Tyr351)

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

570nm/595nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.biossantibodies.com/datasheets/bs-3101R-RBITC

Specificity

This phosphorylation site is found at Tyr345 in Human. Due to the highly conserved nature of this sequence with DOK1, this antibody may react with DOK1 when it is phosphorylated at Tyr362 in Human and Tyr361 in Mouse and Rat.

Gene ID URL

13449

Predicted Cross Reactivity

Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken

Curated Selection

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