Phospho-cdc25C (Ser216) Polyclonal Antibody, Biotin Conjugated
Product Specifications
Background
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Synonyms
CDC25; PPP1R60; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C; CDC25C
Gene ID
995
Swiss Prot
P30307
Modification Site
Ser216
Cellular Locus
Nucleus
Host
Rabbit
Target
Phospho-cdc25C (Ser216)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Biotin
Source
KLH conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Ser216
Applications
WB, ELISA, IHC-P, IHC-F
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C for 12 months.
Specificity
This phosphorylation site is found at Ser173 in Isoform 3 of this protein.
Gene ID URL
995
Predicted Cross Reactivity
Human, Mouse, Rat
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