Phospho-cdc25C (Ser216) Polyclonal Antibody, APC-Cy5.5 Conjugated

Product Specifications

Background

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.

Synonyms

CDC25; PPP1R60; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C; CDC25C

Gene ID

995

Swiss Prot

P30307

Modification Site

Ser216

Cellular Locus

Nucleus

Host

Rabbit

Target

Phospho-cdc25C (Ser216)

Clonality

Polyclonal

Isotype

IgG

Conjugation

APC-Cy5.5

Source

KLH conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Ser216

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/694nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

This phosphorylation site is found at Ser173 in Isoform 3 of this protein.

Gene ID URL

995

Predicted Cross Reactivity

Human, Mouse, Rat

Curated Selection

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