Phospho-cdc25A (Ser178) Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Product Specifications
Background
Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.
Synonyms
Cdc 25a; CDC25A2; Cell division cycle 25 homolog A S. pombe; Cell division cycle 25A; Cell division cycle 25A isoform a; Cell division cycle 25A isoform bv D9Ertd393ev Dual specicity phosphatase CDC25A; EC 3.1.3.48; M phase inducer phosphatase 1; MGC115549.
Gene ID
993
Modification Site
Ser178
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human
Target
Phospho-cdc25A (Ser178)
Clonality
Polyclonal
Isotype
IgG
Conjugation
PerCP-Cy5.5
Source
KLH conjugated synthetic phosphopeptide derived from human cdc25A around the phosphorylation site of Ser178 [QN (p-S) AP]
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
482nm/694nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
993
Predicted Cross Reactivity
Mouse, Rat, Dog, Cow, Rabbit
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