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Phospho-cdc25A (Thr507) Polyclonal Antibody

Product Specifications

Background

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Synonyms

CDC25A2; M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A; CDC25A

Gene ID

993

Swiss Prot

P30304

Modification Site

Thr507

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

Phospho-cdc25A (Thr507)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

KLH conjugated synthetic phosphopeptide derived from human cdc25A around the phosphorylation site of Thr507 [SR (p-T) WA]

Applications

WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Specificity

This phosphorylation site is homologous to Thr497 in Mouse and Thr508 in Rat.

Gene ID URL

993

Predicted Cross Reactivity

Rat, Dog, Cow, Chicken

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