Phospho-CDK1 (Thr14) Polyclonal Antibody, PE-Cy5 Conjugated
Product Specifications
Background
The cell division control protein cdc2, also known as cyclin dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.
Synonyms
P34CDC2; Cdc 2; CDK 1; CDK1; Cell division control protein 2; Cell division control protein 2 homolog; Cell division cycle 2 G1 to S and G2 to M; Cell Divsion Cycle 2 Protein.
Gene ID
983
Modification Site
Thr14
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-CDK1 (Thr14)
Clonality
Polyclonal
Isotype
IgG
Conjugation
PE-Cy5
Source
KLH conjugated synthetic phosphopeptide derived from human cdc2 around the phosphorylation site of Thr14 [EG (p-T) YG]
Applications
WB
Purification
Purified by Protein A.
Excitation Emission
488nm/670nm
Concentration
1µg/µl
Dilution
WB (1:300-5000)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.biossantibodies.com/datasheets/bs-3091R-PE-Cy5
Gene ID URL
983
Predicted Cross Reactivity
Rabbit
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS6347035-01 0.2 mL
SGOL1, ID (SGOL1, SGO1, Shugoshin-like 1, Serologically defined breast cancer antigen NY-BR-85) (FITC)
Ask
View Details 
MBS6347035-02 5x 0.2 mL
SGOL1, ID (SGOL1, SGO1, Shugoshin-like 1, Serologically defined breast cancer antigen NY-BR-85) (FITC)
Ask
View Details 
MBS6362823-01 0.1 mL
VSIG2, CT (VSIG2, CTH, CTXL, V-set and immunoglobulin domain-containing protein 2, Cortical thymocyte-like protein) (MaxLight 405)
Ask
View Details