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Phospho-CEBP alpha (Thr222 + Thr226) Polyclonal Antibody, Cy5 Conjugated

Product Specifications

Background

C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

Synonyms

CEBP; C/EBP-alpha; CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA

Gene ID

1050

Swiss Prot

P49715

Modification Site

Thr226

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-CEBP alpha (Thr222 + Thr226)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy5

Source

KLH conjugated synthetic phosphopeptide derived from human CEBP alpha around the phosphorylation site of Thr226

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

625,650nm/670nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

This phosphorylated site is homologous to that of Thr222 in Mouse and Rat.

Gene ID URL

1050

Predicted Cross Reactivity

Dog, Cow, Pig, Chicken, Rabbit

Curated Selection

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