Phospho-eIF4EBP1 (Thr37 + Thr46) Polyclonal Antibody, APC Conjugated

Product Specifications

Background

Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Synonyms

BP-1; 4EBP1; 4E-BP1; PHAS-I; Eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; EIF4EBP1

Gene ID

1978

Swiss Prot

Q13541

Modification Site

Thr37 + Thr46

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human

Target

Phospho-eIF4EBP1 (Thr37 + Thr46)

Clonality

Polyclonal

Isotype

IgG

Conjugation

APC

Source

KLH conjugated synthetic phosphopeptide derived from human 4EBP1 around the phosphorylation site of Thr37/46

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/660nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

1978

Predicted Cross Reactivity

Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit, Goat

Curated Selection

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