Phospho-14-3-3 protein zeta (Ser58) Polyclonal Antibody, Cy3 Conjugated
Product Specifications
Background
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions.
Synonyms
YWHAEphospho S58; YWHAZphospho S58; 14 3 3; 14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta; 14-3-3 protein beta/alpha; KCIP 1; Protein 1054; Protein kinase C inhibitor protein 1; YWHAB; YWHAZ; GW128; HS1; KCIP-1; YWHAE; YWHAA; 1433Z_HUMAN; Full=14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1.
Gene ID
7534, 7529, 10971
Modification Site
Ser58
Cellular Locus
Cytoplasm
Host
Rabbit
Cross Reactivity
Human
Target
Phospho-14-3-3 protein zeta (Ser58)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy3
Source
KLH conjugated synthetic phosphopeptide derived from human 14-3-3 protein zeta/delta around the phosphorylation site of Ser58 [RS (p-S) WR]
Applications
WB, FCM
Purification
Purified by Protein A.
Excitation Emission
512,550nm/570,615nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Specificity
This antibody will react with all 14-3-3 proteins containing the conserved motif RS (S-p) WR.
Gene ID URL
7534, 7529, 10971
Predicted Cross Reactivity
Mouse, Rat, Dog, Cow, Horse, Chicken, Rabbit
Curated Selection
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