Phospho-IKB alpha (Ser32 + Ser36) Polyclonal Antibody

Product Specifications

Background

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Synonyms

IKBA; MAD-3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; MAD3

Gene ID

4792

Swiss Prot

P25963

Modification Site

Ser32/36

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-IKB alpha (Ser32 + Ser36)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

KLH conjugated synthetic phosphopeptide derived from human IKB alpha around the phosphorylation site of Ser32/36

Applications

WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Specificity

These phosphorylation sites are homologous among the species listed.

Gene ID URL

4792

Predicted Cross Reactivity

Dog, Cow, Sheep, Pig, Chicken, Rabbit

Available Sizes

Curated Selection

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