Phospho-eIF4E (Ser209) Polyclonal Antibody, AbBy Fluor™ 680 Conjugated

Product Specifications

Background

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Synonyms

CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

Gene ID

1977

Swiss Prot

P06730

Modification Site

Ser209

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat, Zebrafish

Target

Phospho-eIF4E (Ser209)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 680

Source

KLH conjugated synthetic phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209 [SG (p-S) TT]

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

679nm/702nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

1977

Predicted Cross Reactivity

Dog, Cow, Pig, Horse, Chicken, Rabbit

Curated Selection

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