Anti-Shank 1 Antibody

Mouse Monoclonal Antibody specific to Shank1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Shank1, GKAP/SAPAP-interacting protein, SPANK-1, Somatostatin receptor-interacting protein, SSTR-interacting protein, SSTRIP, Synamon

Gene Name

Shank1

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=Shank1">Shank1</a>

UniProt

Q9WV48

Cellular Locus

Cytoplasm, Cell junction, synapse, Cell junction, synapse, postsynaptic density

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 469-691 (SH3/PDZ domains) of rat Shank1 (accession no. Q9WV48). This sequence is 96

Target Antigen

SH3 and multiple ankyrin repeat domains protein 1

Target

Shank 1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, AM

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. Overexpression promotes maturation of dendritic spines and the enlargement of spine heads via its ability to recruit Homer to postsynaptic sites, and enhances presynaptic function. {PubMed:11498055, PubMed:18287537}.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. Bands of ~190-220kDa (alternative splice variants) are detected.Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/mL Immunofluorescence: use at 1-10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Adult rat brain.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat Shank 1. It does not react with Shank2 or 3.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Shank proteins are a family of scaffold proteins identified through their interaction with a variety of membrane and cyto- plasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are crucial in receptor tyrosine kinase signaling. Recent studies suggest that disruption of glutamate receptors at the Shank-postsynaptic platform could contribute to the destruction of post- synaptic density which underlies synaptic dysfunction and loss in Alzheimer's disease. Shank1 might be relevant to human autism spectrum disorders due to its differential role in specific cognitive processes along with its importance in synapse structure and function.