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Beta tubulin Polyclonal Antibody, Cy3 Conjugated

Product Specifications

Background

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29 (1) :53-60) and therefore beta Tubulin should not be used as loading control for these tissues.

Synonyms

Beta 4 tubulin; Tubulin-beta; Tubulin beta; Beta 5 tubulin; BetaTubulin; Beta-Tubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; Tubulin beta-5 chain; TUBB4A; TUBB4; Tubulin 5 beta; Tubulin beta-4 chain; TBB4A_HUMAN; Tubulin beta-4A chain.

Gene ID

203068

Swiss Prot

P07437

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Immunogen

61-160/444

Target

Beta tubulin

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy3

Source

KLH conjugated synthetic peptide derived from human Beta tubulin

Applications

WB, FCM

Purification

Purified by Protein A.

Excitation Emission

512,550nm/570,615nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

203068

Predicted Cross Reactivity

Pig, Rabbit

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