HIF-1 Alpha Polyclonal Antibody, Biotin Conjugated
Product Specifications
Background
Functions as a master transcriptional regulator of theadaptive response to hypoxia. Under hypoxic conditions activatesthe transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelialgrowth factor, and other genes whose protein products increaseoxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumorangiogenesis and pathophysiology of ischemic disease. Binds to coreDNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment oftranscriptional coactivators such as CREBPB and EP300. Activity isenhanced by interaction with both, NCOA1 or NCOA2. Interaction withredox regulatory protein APEX seems to activate CTAD andpotentiates activation by NCOA1 and CREBBP.
Synonyms
HIF-1-alpha; hypoxia-inducible factor 1, alpha subunit isoform 1; Hypoxia-inducible factor 1 alpha; HIF-1 alpha; HIF1 alpha; HIF-1a; HIF-1alpha; ARNT-interacting protein; Member of PAS protein1; Basic-helix-loop-helix-PAS protein MOP1; Hypoxia-inducible factor -1 Alpha.
Gene ID
3091
Swiss Prot
Q16665
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Rat
Immunogen
661-760/826
Target
HIF-1 Alpha
Clonality
Polyclonal
Isotype
IgG
Conjugation
Biotin
Source
KLH conjugated synthetic peptide derived from human HIF-1 Alpha
Applications
WB, ELISA, IHC-P, IHC-F
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C for 12 months.
Gene ID URL
3091
Predicted Cross Reactivity
Mouse, Chicken
Curated Selection
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