Phospho-SMAD5 (Ser463 + Ser465) Polyclonal Antibody, Cy5 Conjugated
Product Specifications
Background
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD) .
Synonyms
Mothers against decapentaplegic homolog 5; SMAD5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5
Gene ID
4090
Swiss Prot
Q99717
Modification Site
Ser463 + Ser465
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Target
Phospho-SMAD5 (Ser463 + Ser465)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy5
Source
KLH conjugated synthesised phosphopeptide derived from human SMAD5 around the phosphorylation site of Ser463 + Ser465
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
625,650nm/670nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
4090
Predicted Cross Reactivity
Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Rabbit, GuineaPig
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