Phospho-SMAD5 (Ser463 + Ser465) Polyclonal Antibody
Product Specifications
Background
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD) .
Synonyms
Mothers against decapentaplegic homolog 5; SMAD5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5
Gene ID
4090
Swiss Prot
Q99717
Modification Site
Ser463 + Ser465
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Target
Phospho-SMAD5 (Ser463 + Ser465)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthesised phosphopeptide derived from human SMAD5 around the phosphorylation site of Ser463 + Ser465
Applications
WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
4090
Predicted Cross Reactivity
Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Rabbit, GuineaPig
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