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SAE1 Polyclonal Antibody, Cy5.5 Conjugated

Product Specifications

Background

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Synonyms

SUMO-activating enzyme subunit 1; SAE1; Ubiquitin-like 1-activating enzyme E1A; SAE1; AOS1; SUA1; UBLE1A

Gene ID

10055

Swiss Prot

Q9UBE0

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Mouse

Immunogen

1-100/346

Target

SAE1

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy5.5

Source

KLH conjugated synthetic peptide derived from human SAE1, Isoform 2

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

675nm/694nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

10055

Predicted Cross Reactivity

Human, Rat

Curated Selection

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