Rad52 Polyclonal Antibody
Product Specifications
Background
DNA double-strand breaks can be generated by exposure to ionizing radiation or through endogenous cellular reactions. Double-strand breaks are generally thought to be repaired via one of two distinct mechanisms: homologous recombination (HR) or non-homologous end joining (NHEJ) . Homologous recombination requires the activity of the members of the RAD52 epistasis group, which include RAD50, RAD51, RAD52, RAD54, RDH54/TID1, RAD55, RAD57, RAD59, MRE11, and XRS2. RAD52 can catalyze strand annealing reactions, and can form oligomers that attach to ends of single-stranded DNA.
Synonyms
DNA repair protein RAD52; DNA repair protein RAD52 homolog; RAD 52; RAD52 homolog S. cerevisiae; RAD52 homolog; Recombination protein RAD52; Rhabdomyosarcoma antigen MU RMS 40.23.
Gene ID
5893
Cellular Locus
Nucleus
Host
Rabbit
Immunogen
101-200/418
Target
Rad52
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human Rad52
Applications
ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
5893
Predicted Cross Reactivity
Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit
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