SPERT Polyclonal Antibody, Cy5.5 Conjugated
Product Specifications
Background
A variety of morphological and molecular changes are required for spermatozoa formation. These steps are temporally guided by the transcription and translation of several testis-specific genes. SPERT (spermatid associated), also known as CBY2 (chibby homolog 2), spermatid flower-like structure protein or NURIT, is a 448 amino acid novel leucine-zipper protein belonging to the chibby family of proteins.Expressed uniquely in the spermatid flower-like structure, SPERT interacts with Nek1, a member of the NIMA-family kinase family that is associated centrosomal stability and ciliogenesis. Containing a leucine-zipper motif and two coiled-coil regions, SPERT is transcribed through the elongation stage of the spermatids. SPERT is absent from mature spermatozoa and is thought to be involved in transporting proteins that are to be discarded via the residual bodies.
Synonyms
Spermatid-associated protein; SPERT; Protein chibby homolog 2; SPERT; CBY2
Gene ID
220082
Swiss Prot
Q8NA61
Cellular Locus
Cytoplasm
Host
Rabbit
Cross Reactivity
Human, Rat
Immunogen
1-100/448
Target
SPERT
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy5.5
Source
KLH conjugated synthetic peptide derived from human SPERT.
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
675nm/694nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
220082
Predicted Cross Reactivity
Mouse, Cow, Sheep, Horse, Rabbit
Curated Selection
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