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DNA Ligase IV Polyclonal Antibody, AbBy Fluor™ 750 Conjugated

Product Specifications

Background

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V (D) J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Synonyms

LIG4S; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4; LIG4

Gene ID

3981

Swiss Prot

P49917

Host

Rabbit

Cross Reactivity

Human

Immunogen

51-150/911

Target

DNA Ligase IV

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 750

Source

KLH conjugated synthetic peptide derived from human DNA Ligase IV

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

749nm/775nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.biossantibodies.com/datasheets/bs-1677R-BF750

Gene ID URL

3981

Predicted Cross Reactivity

Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken, Rabbit

Curated Selection

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