KLB Polyclonal Antibody
Product Specifications
Background
BetaKlotho is a 1044 amino acid single-pass type III membrane protein that plays a key role in bile acid and cholesterol metabolism by suppressing transcription of CYP7A1 (cholesterol 7-alpha-hydroxylase), the rate-limiting enzyme in bile acid synthesis. Homozygous negative betaKlotho mice showed dramatically elevated bile acid synthesis and secretion, as well as a strong upregulation of CYP7A1 and CYP8B1 and resistance to gallstone formation. FGF19 and FGF21 require direct interaction with betaKlotho for activation, intracellular signaling and gene expression modulation. Both Klotho and betaKlotho consist of two internal repeats similar to family 1 glycosidases. BetaKlotho contains two glycosyl hydrolase 1 regions, however since these regions lack essential glutamic acid residues at specific and crucial locations, the domains appear to be inactive.
Synonyms
Beta-klotho; BetaKlotho; BKL; KLB; KLOTB_HUMAN; Klotho beta like protein; Klotho beta-like protein.
Gene ID
152831
Swiss Prot
Q86Z14
Host
Rabbit
Cross Reactivity
Human
Immunogen
451-550/1044
Target
KLB
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human KLB
Applications
WB, ELISA, IHC-P, IHC-F, IF, ICC/IF
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (WB=1:500-2000), ELISA (ELISA=1:5000-10000), IHC-P (IHC-P=1:100-500), IHC-F (IHC-F=1:100-500), IF (IF=1:100-500), ICC/IF (ICC/IF=1:100-500)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C for 12 months.
Gene ID URL
152831
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