Phospho-ERK1 (Thr202/Tyr204) + ERK2 (Thr183/Tyr185) Polyclonal Antibody, PE Conjugated
Product Specifications
Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Synonyms
ERK; p38; p4; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; Extracellular signal-regulated kinase 2; MAP kinase isoform p42; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1
Gene ID
55945595
Swiss Prot
P28482
Modification Site
Thr183/Tyr185
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-ERK1 (Thr202/Tyr204) + ERK2 (Thr183/Tyr185)
Clonality
Polyclonal
Isotype
IgG
Conjugation
PE
Source
KLH conjugated synthetic phosphopeptide derived from human ERK2 around the phosphorylation site of Thr183/Tyr185
Applications
WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
496,564nm/578nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
55945595
Predicted Cross Reactivity
Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig
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