Phospho-FAK (Tyr577) Polyclonal Antibody, AbBy Fluor™ 555 Conjugated
Product Specifications
Background
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. [SUBCELLULAR LOCATION] Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Constituent of focal adhesions.
Synonyms
FAK phospho Y577; p-FAK phospho Y577; FADK 1; FADK; FAK 1; FAK related non kinase polypeptide; FAK1; Focal adhesion kinase 1; FRNK; pp125FAK; Protein tyrosine kinase 2; Protein Tyrosine Kinase Cytoplasmic; PTK 2; FAK1_HUMAN; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK.
Gene ID
5747
Modification Site
Tyr577
Cellular Locus
Cytoplasm, Nucleus, Cell membrane
Host
Rabbit
Cross Reactivity
Mouse
Target
Phospho-FAK (Tyr577)
Clonality
Polyclonal
Isotype
IgG
Conjugation
AbBy Fluor™ 555
Source
KLH conjugated synthetic phosphopeptide derived from human FAK around the phosphorylation site of Tyr577
Applications
WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
553nm/568nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
5747
Predicted Cross Reactivity
Human, Rat
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
DNM-SP23-038 5 mL
DiagPoly™ Goat Anti-Mouse IgG (H&L) Fluorescent Polystyrene Particles, Nile Red, 0.4-0.6 μm
Ask
View Details 
125712-ML650 100 µL
DDIT4L (REDD2, RTP801L, DNA Damage-inducible Transcript 4-like Protein, HIF-1 Responsive Protein RTP801-like, Protein Regulated in Development and DNA Damage Response 2) (MaxLight 650)
Ask
View Details