Tris Tricine SDS Buffer 10X Solution

<strong>Tris-Tricine-SDS Buffer 10X</strong>_x000D_ <strong>Catalog number:</strong> B2010112_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Volume/Weight:</strong> 100 mL_x000D_ <strong>pH:</strong> 8.2_x000D_ <strong>Supplied as:</strong> 10X concentrate (dilute to 1X with di-water before use)_x000D_ <strong>Appearance:</strong> Clear solution_x000D_ <strong>Applications:</strong> running buffer for polyacrylamide based separation of bronchoalveolar lavage fluids as well as low molecular weight proteins._x000D_ <strong>Storage:</strong> Room temperature_x000D_ <strong>Keywords:</strong> Tris-Tricine-SDS, TTS, Low molecular weight SDS-PAGE_x000D_ <strong>Grade:</strong> Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Fernandez-Patron C, Hardy E, Sosa A, Seoane J, Castellanos L. Double staining_x000D_ of coomassie blue-stained polyacrylamide gels by imidazole-sodium dodecyl_x000D_ sulfate-zinc reverse staining: sensitive detection of coomassie blue-undetected_x000D_ proteins. Anal Biochem. 1995 Jan 1;224(1):263-9._x000D_ _x000D_ 2: Brum AM, Thomas AD, Sabeur K, Ball BA. Evaluation of Coomassie blue staining_x000D_ of the acrosome of equine and canine spermatozoa. Am J Vet Res. 2006_x000D_ Feb;67(2):358-62._x000D_ _x000D_ 3: Marshall T, Williams KM. Total protein determination in urine: elimination of_x000D_ a differential response between the coomassie blue and pyrogallol red protein_x000D_ dye-binding assays. Clin Chem. 2000 Mar;46(3):392-8._x000D_ _x000D_ 4: Lim CW, Chisnall WN, Stokes YM, Pratt R, Crooke MJ. Effects of sodium_x000D_ dodecylsulphate, dye concentration and paraprotein on coomassie blue dye-binding_x000D_ assays for protein in urine. Clin Biochem. 1988 Oct;21(5):277-81._x000D_ _x000D_ 5: Fujita T, Toda T, Ohashi M. Enzyme-linked immunodetection of proteins on_x000D_ Coomassie blue-stained two-dimensional cellulose acetate membranes. Anal_x000D_ Biochem. 1986 Nov 15;159(1):8-11._x000D_ _x000D_ 6: Fountoulakis M, Juranville JF, Manneberg M. Comparison of the Coomassie_x000D_ brilliant blue, bicinchoninic acid and Lowry quantitation assays, using non-_x000D_ glycosylated and glycosylated proteins. J Biochem Biophys Methods. 1992_x000D_ Jun;24(3-4):265-74._x000D_ _x000D_ 7: Spector T. Refinement of the coomassie blue method of protein quantitation. A_x000D_ simple and linear spectrophotometric assay for less than or equal to 0.5 to 50_x000D_ microgram of protein. <a href="https://pubmed.ncbi.nlm.nih.gov/655375/">Anal Biochem</a>. 1978 May;86(1):142-6._x000D_ _x000D_ <strong>Products Related to Tris-Tricine-SDS Buffer 10X</strong>: <a href="https://moleculardepot.com/product-category/buffers/">Buffers and Solutions</a>