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Phospho-alpha Adducin (Ser436) Polyclonal Antibody, APC Conjugated

Product Specifications

Background

Adducins are a family of cytoskeleton proteins encoded by three genes (Alpha, Beta, Gamma) . Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and Adducins are central in the cortical actin network organization. Adducin Alpha is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin Alpha and Adducin Gamma is ubiquitous in contrast to the restricted expression of Adducin Beta. Adducin Beta is expressed at high levels in brain and hematopoietic tissues, such as bone marrow, in humans, and in spleen in mice.

Synonyms

Alpha Adducinphospho S436; alpha Adducinphospho Ser436; p-alpha AdducinS436; p-alpha AdducinSer436; ADD 1; ADD A; ADD1; ADD1 protein; ADDA; ADDA_HUMAN; Adducin 1 alpha; Adducin 1 alpha; Alpha adducin; Alpha-adducin; Erythrocyte adducin alpha subunit; Erythrocyte hosphor subunit alpha; Erythrocyte adducin subunit alpha.

Gene ID

118

Modification Site

Ser436

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

Phospho-alpha Adducin (Ser436)

Clonality

Polyclonal

Isotype

IgG

Conjugation

APC

Source

KLH conjugated synthetic phosphopeptide derived from human alpha Adducin around the phosphorylation site of Ser436

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/660nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

118

Predicted Cross Reactivity

Rat, Dog, Cow, Horse, Chicken

Curated Selection

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