DFHBI-1T

DFHBI-1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Displays peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. DFHBI-1T has been found to have lower background fluorescence than DFHBI (Cat. No. B2797) and exhibits an overall increase in brightness in living cells. DFHBI-1T is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Spinach™, Spinch2™, and Broccoli™ RNA tags.