Phospho-EIF4B (Ser406) Polyclonal Antibody, FITC Conjugated
Product Specifications
Background
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Synonyms
Eukaryotic translation initiation factor 4B; EIF4B; eIF-4B
Gene ID
1975
Swiss Prot
P23588
Modification Site
Ser406
Cellular Locus
Cytoplasm
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-EIF4B (Ser406)
Clonality
Polyclonal
Isotype
IgG
Conjugation
FITC
Source
KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Ser406
Applications
WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
494nm/518nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
1975
Predicted Cross Reactivity
Dog, Cow, Sheep, Horse
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