Phospho-EIF4B (Ser406) Polyclonal Antibody, AbBy Fluor™ 647 Conjugated

Product Specifications

Background

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Synonyms

Eukaryotic translation initiation factor 4B; EIF4B; eIF-4B

Gene ID

1975

Swiss Prot

P23588

Modification Site

Ser406

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-EIF4B (Ser406)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 647

Source

KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Ser406

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/665nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

1975

Predicted Cross Reactivity

Dog, Cow, Sheep, Horse

Curated Selection

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