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Human Activin B Protein, His tag (Animal-Free)

Activin B is the cytokine of the TGF-beta family, which is a 13 kDa protein containing 115 amino acid residues. Activin B has the wide variety of biological function including stem cell differentiation, inflammation, bone remodeling and neural development. They also have a role in regulation of FSH secretion. Activin B induces the phosphorylation of Smad which binds the smad binding element.

Product Specifications

Background

Activin B is the cytokine of the TGF-beta family, which is a 13 kDa protein containing 115 amino acid residues. Activin B has the wide variety of biological function including stem cell differentiation, inflammation, bone remodeling and neural development. They also have a role in regulation of FSH secretion. Activin B induces the phosphorylation of Smad which binds the smad binding element.

Product Name Alternative

Activin Beta B, INHBB; inhibin beta B chain

Gene ID

3625

Accession Number

P09529

Tag

Activin B

Type

Cytokines and proteins

Applications

His tag

Purity

> 98 % as determined by SDS-PAGE

Activity

Measure by its ability to induce hemoglobin expression in K562 cells. The ED₅₀ for this effect is <0.7 ng/mL. The specific activity of recombinant human Activin B is > 1.5 x 10⁶ IU/mg.

Molecular Formula

13.5 kDa

Molecular Weight

13.5 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin : < 0.1 EU per μg of the protein as determined by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA) . Please prevent freeze-thaw cycles.

Protein Length

The recombinant Human Activin B Protein consists of 115 amino acids and predicts a molecular mass of 13.5 kDa.

Formulation

Lyophilized from sterile PBS, pH 8.0.

Applications Notes

Human

Other Product Names

Activin B, ActivinB, Activin-B, Activin Beta B, INHBB; inhibin beta B chain

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.

Preparation

E.coli

Accession Number URL

https://www.uniprot.org/uniprotkb/P09529/entry

AA Sequence

Amino acid sequence derived from Human Activin B Protein (Gly293-Ala407) (P09529) was expressed with 6xHis tag at the C-terminus.

Available Sizes

Frequently Asked Questions

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