Phospho-14-3-3 beta + zeta (Ser186 / Ser184) Polyclonal Antibody

Product Specifications

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions.

Synonyms

14-3-3 beta + zeta (phospho S184 + S186) ; 14-3-3 beta + zeta (phospho S186) ; 14-3-3 beta + zeta (phospho Ser186) ; p-14-3-3 beta + zeta (Ser186) 14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta/delta; 14 3 3 zeta; HS1; KCIP1; YWHAB; YWHAZ.

Gene ID

7529

Swiss Prot

P31946

Modification Site

Ser186 / Ser184

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-14-3-3 beta + zeta (Ser186 / Ser184)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

KLH conjugated synthesised phosphopeptide derived from human 14-3-3 beta + zeta around the phosphorylation site of Ser186 / Ser184

Applications

WB, ELISA, FCM, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

WB (1:300-5000), ELISA (1:500-1000), FCM (1:20-100), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)