Phospho-EEF2k (Ser359) Polyclonal Antibody, APC Conjugated
Product Specifications
Background
Eukaryotic elongation factor 2 kinase (EEF2k) previously known as Ca2+/calmodulin dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor 2 (eEf2) . Phosphorylation of eEF2 by eEF2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation. The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively via phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK) . There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors.
Synonyms
EEF2K phospho S359; p-EEF2K phospho S359; Calcium/calmodulin dependent eukaryotic elongation factor 2; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase; Calmodulin dependent protein kinase III; cb365; eEF 2 kinase; eEF 2K; EEF2K protein; Elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; kinase eEF2K; SMEF2K; EF2K_HUMAN.
Gene ID
29904
Modification Site
Ser359
Cellular Locus
Cytoplasm
Host
Rabbit
Target
Phospho-EEF2k (Ser359)
Clonality
Polyclonal
Isotype
IgG
Conjugation
APC
Source
KLH conjugated synthetic phosphopeptide derived from human EEF2k around the phosphorylation site of Ser359
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
650nm/660nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
29904
Predicted Cross Reactivity
Human, Mouse, Rat, Dog, Sheep, Horse, Chicken, Rabbit
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
